アブラヤシから得られる植物油の脂肪酸から調製されるトレハロース脂肪酸エステルの利用性開拓

Abstract

The oil derived from the oil palm plant has become one of the most contested agricultural commodities in Southeast Asia, and development of novel uses for it is in high demand. Trehalose fatty acid esters have begun attracting attention due to their specific potent applications (based on the exceptional intrinsic properties of the hydrophilic trehalose part) and their amphiphilicity. In order to enhance the usability of palm products and trehalose fatty acid esters, in this present study we focused on the use of trehalose fatty acid monoesters (TMEs) prepared with natural fatty acids extracted from oil palms as cryoprotectants. Both saturated fatty acid and unsaturated fatty acid (e.g., oleic acid) could be used as the lipid substrate for the solvent-free enzymatic synthesis of trehalose fatty acid esters, using Novozym®435 as the enzyme catalyst. Additionally, three pure TMEs with fatty acid substitutions (e.g., lauric acid, palmitic acid, oleic acid, and mixtures of these) were assessed for their ability to maintain the enzymatic activity of lactate dehydrogenase (LDH) in the freeze/thaw processes. While 6-O-dodecanoyl trehalose (TME12) exhibited a much higher effect for preserving LDH activity than 6-O-hexadecanoyl trehalose (TRE16), substituting TME12 with TRE16 retained LDH activity up to a molar ratio of about 1:1 caused no distinct loss in the LDH’s enzymatic activity, which indicates the effectiveness of a mixing strategy for the stabilizer, and that the concept is important for the use of TME product prepared with natural fatty acid extracted from oil palms.